小麦八氢番茄红素合成酶家族的生物信息学分析及表达模式检测

摘要：

八氢番茄红素合成酶(phytoene synthase,PSY)是植物中类胡萝卜素合成途径的限速酶.提高 PSY 基因的表达量有助于提升植株中类胡萝卜素含量,进而提高植物的光合作用和籽粒品质,但小麦(Triticum aestivum)中有关 PSY 的研究报道较少,以拟南芥和水稻 PSY 蛋白序列为模板,从小麦数据库中筛选到9个同源蛋白序列,通过生物信息学技术分析后发现,9个小麦PSY蛋白(TaPSYs)可聚为3组,均为疏水性蛋白,皆定位于叶绿体,都含有SQS-PSY 基序,基序内以 《-螺旋结构为主.分析 PSY 基因的启动子序列,发现含有脱落酸(abscisic acid,ABA)、水杨酸(salicvlic acid,SA),茉莉酸甲酯(methyliasmonate,MeJA)等激素的响应元件和逆境响应元件,对小麦幼苗分别用ABA、SA、MeJA 和聚乙二醇 6000(polyethylene glycol6000,PEG6000)分别处理6h后,RT-gPCR检测叶片中 TaP-SY1-6的表达量,发现都显著下调,但 TaPSY7-9基因的表达水平在不同品种的小麦中差异较大,认为 TaPSY7-9基因的表达水平与品种间的抗性差异有关,本研究对小麦PSY蛋白家族的生物信息学分析为深人探讨小麦 PSY的功能提供了理论参考,对小麦 PSY 基因家族的表达模式分析为小麦抗性育种提供新的候选基因。

Phvtoene synthase(PSY) is a rate limiting enzyme in the carotenoid synthesis pathway in plants.Increasing the expression level of PSY gene helps to increase the carotenoid content in plants, thereby improving photosynthesis and grain quality, However, there is still a significant gap in research on PSY in wheat(Triticum aestivum). Here, the PSY protein sequences of Arabidopsis and rice were used as templates and screened 9 homologous sequences from a wheat database, Through bioinformatics analysis, it was found that they can be clustered into three groups, all of which are hydrophobic proteins located in chloroplasts and contain SQS-PSY motifs. a-helix is dominant in secondary structure of PSY in wheat, After analyzing the cis-acting element, the hormone responsive elements such as response to abscisic acid(ABA), salicylic acid(SA), methyl jasmonate(MelA), and stress responsive elements were found in promoters of TaPSY genes. R'T-gPCR technique was used to detect the expression levels of TaPSY1-6 in the leaves of wheat seedlings treated with ABA, SA, MejA, and polyethylene glycol 6000(PEG6000) for 6 hours, respectively. It was found that they were significantly downregulated, However, the expression levels of TaPSy7-9 varied greatly among wheat varieties, suggesting that the expression levels of TaPSY7-9 are related to the stress resistance differences between varieties. The bioinformatics analysis of the wheat PSY protein family in this study pro- vides a theoretical reference for further exploring the function of wheat PSY, and the expression profile analysis of the wheat PSY gene family provides new candidate genes for wheat resistance breeding.

作者:

石永春，乔占锋，卢了一，李欢欢

Shi Yongchun，Qiao Zhanfeng， Lu Liaoyi，Li Huanhuan

机构地区：

河南农业大学生命科学学院；河南省人民胜利渠保障中心

引用本文：

石永春，乔占锋，卢了一等。小麦八氢番茄红素合成酶家族的生物信息学分析及表达模式检测[ J ].河南师范大学学报(自然科学版),2025,53(5):16-23.(Shi Yongchun,Qiao Zhanfeng,Lu Liaoyi,et al,Bioinformatic identification and expression profile analysis of PSY gene family in wheat[ J ].Journal of Henan Normal University(Natural Science Edition),2025,53(5):16-23.D01:10.16366/j.cnki1000-2367.2024.01.10.0003.)

基金：

国家自然科学基金；河南省人民胜利渠保障中心科研项目

关键词：

小麦；PSY；生物信息；启动子；表达分析

wheat; PSY;bioinformatics;promoter; expression analysis

分类号：

Q527

小麦八氢番茄红素合成酶家族的生物信息学分析及表达模式检测.pdf