细叶远志皂苷抑制 NF-KB 信号通路发挥抗抑郁作用研究

摘要：

[目的]探究细叶远志皂苷(TEN)对 CUMS抑郁模型小鼠的治疗功效及机制。[方法]60只体质量18~22g的8周龄的 C57BL/6 雄性小鼠随机均分为对照组(Control)、抑郁模型组(CUMS)、低剂量TEN组(TEN-L)、中剂量TEN 组(TEN-M),高剂量TEN组(TEN-H)和氟西汀组(Flu),除 Contrl组外,其余5组随机给予不可预知性轻度刺激8周,其中 TEN-L,TEN-M、TEN-H 和 Flu组小鼠于第5~8周分别灌胃给药，第9周采用糖水偏好实验(SPT)、悬尾实验(TST)、旷场实验(OFT)和 Morris 水迷宫实验(MWMT)评价小鼠的抑郁行为,苏木素伊红(HE)染色观察小鼠海马组织结构,酶联免疫吸附(ELISA)实验检测海马组织1L-1β、1L-6和 TNF-α水平,免疫印迹(WB)检测海马组织p-IKKβ/IKKβ、p-IxBa/IκBα 和 p-NF-κB/NF-κB 的表达比率。[结果]与CUMS组相比,TEN-M、TEN-H和 Flu组小鼠的糖水偏好率上升，悬尾不动时间减少，旷场内活动的总路程、中心区路程及中心区活动时间增多，水迷官内的潜伏时间减少和目标象限活动路程增多，海马组织的神经元损伤减轻,1L-1β、1L-6和 TNF-α含量下降pp-IKKβ/IKKβ、p-IxBa/IκBα 和 p-NF-κB/NF-κB 蛋白的表达均降低。[结论]TEN 能通过抑制 NF-κB 信号通路,降低抑郁小鼠海马组织的炎症水平和神经元损伤，减轻其抑郁行为。

[Objective]To investigate the therapeutic efficacy and mechanism of tenuifolin(TEN) on chronic unpredictability mild stress(CUMS) depression model mice. [Methods]Sixty C57BL/6 male mice weighing 18-22g aged 8 week were randomly divided into control group( Control), CUMS group(CUMS), low-dose TEN group( TEN-L), medium-dose TEN group(TEN-M), high-dose TEN group(TEN-H) and fluoxetine group( Flu). Besides the control group, the other 5 groups were given CUMS for 8 weeks, among which the TEN-L, TEN-M, TEN-H and Flu groups were given intragastric administration at 5-8th week, respectively. At the 9th week, the depression behavior of mice was evaluated by the sugar preference test (SPT), tail suspension test(TST), open field test(OFT), and Morris water maze test( MWMT), the hippocampus structure of mice was detected by hematoxylin-eosin(HE) staining, the levels of lL-1β, lL-6 andTNF-α in hippocampus were detected by enzyme-linked immunosorbent assay(ELISA), and the ratio of p-IKKβ/lKKβ, p-IκBα/IκBα and p-NF-κB/NF-κB in hippocampus was detected by western blot(WB). [Results]Compared with CUMS group, the sugar water preference rate of TEN-M TEN-H and Flu groups inereased, the tail suspension immobility time decreased, the total distance,the distance of central area and the activity time in central area increased in the open field, the escape latency time reduced and the target quadrant activity distance increased in the Morris water maze, neuron damage in the hippocampus believed, the levels of lL-1β, lL-6 andTNF-αdeclined. the ratio of p-lKKβ/IKKβ, p-IκBα/IκBα and p-NF-κB/NF-κB decreased. [Conclusion] TEN can decrease the level of inflammation and neuronal damage in hippocampus of depressed mice by inhibiting NF-κB signaling pathway, and ultimately alleviate depressive behavior in depressed mice.

作者:

唐超智，翟自晗，张玉玲，李一晨

Tang Chaozhi，Zhai Zihan，Zhang Yuling， Li Yichen

机构地区：

六盘水师范学院生物科学与技术学院；河南师范大学生命科学学院

引用本文：

唐超智，翟自晗，张玉玲等,细叶远志皂苷抑制NF-κB信号通路发挥抗抑郁作用研究[J].河南师范大学学报(自然科学版),2025,53(6):117-123.(Tang Chaozhi,Zhai Zihan,Zhang Yuling,et al.Study on the antidepressant efficacy of tenuifolin by inhibiting NF-κB signaling pathway[J].Journal of Henan Normal University( Natural Science Edition),2025,53(6):117-123.DOI:10.16366/j.cnki1000-2367.2023.09.14.0002.)

基金：

国家自然科学基金

关键词：

细叶远志皂苷；抑郁；NF-kB信号通路；炎症

tenuifolin; depression; nuclear factor-κB signaling pathway; inflammation

分类号：

R285.5