miR-324-5p/HuR轴调控前列腺癌细胞增殖的分子机制研究

摘要：

探究前列腺癌细胞PC-3中miR-324-5p调控HuR的表达及其介导PC-3的增殖和凋亡,通过West-ermn Blot和RT-PCR检测在PC-3细胞中高表达或抑制 miR-324-5p、敲降HuR后的凋亡相关蛋白和基因的表达水平,通过CCK-8检测 miR-324-5p或Inhibitor,HuR siRNA干扰处理后PC-3的细胞增殖水平,通过荧光素酶报告基因系统论证 miR-324-5p结合HuR的3UTR的特定区域并调控其活性.结果发现:miR-324-5p可能通过结合HuR的3UTR区域,调控BAX、Bcl-2和Cleaved Caspase3的表达水平,从而显著降低前列腺癌细胞的增殖并促进其凋亡.研究为前列腺癌的发病机制提供了新的见解,为研发治疗药物提供了新思路.

This study aimed to investigate the regulation of HuR expression by miR-324-5p and its role in mediating the proliferation and apoptosis of PC-3 prostate cancer cells. The expression levels of apoptosis-related proteins and genes were de-tected by Wester Blot and RT-PCR after miR-324-5p overexpression or inhibition, or after HuR knockdown in PC-3 cells. Cell proliferation was assessed using the CCK-8 assay following treatment with miR-324-5p mimic or inhibitor, or HuR siRNA.The binding of miR-324-5p to a specific region of the HuR Suntranslated region (3UTR) and its regulatory effect on HuR activ-ity were confirmed using a luciferase reporter gene system. The results demonstrated that miR-324-5p potentially binds to the 3UTR of HuR, modulates the expression levels of BAX, Bcl-2, and Cleaved Caspase-3, significantly inhibits the proliferation of prostate cancer cells, and promotes their apoptosis, This research provides new insights into the pathophysiology of prostate cancer and suggests a novel strategy for the development of therapeutic agents.

作者:

景亚男，郑俊斌，李沐天，李西

Jing Yanan,Zheng Junbin,Li Mutian,Li Xi

机构地区：

温州医科大学附属康宁医院；浙江省精神卫生临床医学研究中心；玉环市人民医院泌尿外科；.温州肯恩大学理学院

引用本文：

景亚男，郑俊斌，李沐天等。mR-324-5p/HuR轴调控前列腺癌细胞增殖的分子机制研究[J].河南师范大学学报(自然科学版),2026,54(1):114-120.(Jing Yanan,Zheng Junbin,Li Mutian,et al.Molecular mechanisms of the miR-324-5p/ HuR axis in regulating prostate cancer cell proliferation[J].Journal of Henan Normal University(Natural Science Edition),2026,54(1):114-120.DOI:10.16366/j.cnki.1000-2367.2025.06.04.0001.)

基金：

国家自然科学基金；台州市科技局项目

关键词：

前列腺癌；PC-3细胞；miR-324-5p；HuR；细胞增殖；凋亡

prostate cancer; PC-3 cells; miR-324-5p; HuR; cell proliferation; apoptosis

分类号：

Q513.2

miR-324-5p/HuR轴调控前列腺癌细胞增殖的分子机制研究.pdf