环境因子介导细胞表面特性解析铜绿微囊藻群体形成机制

摘要：

群体的形成对于微囊藻种群优势维持和水华暴发至关重要.以铜绿微囊藻为研究对象，探究其在不同Ca²⁺、低N、P质量浓度以及温度和光照强度下的群体形成情况及机理.结果表明，在高Ca²⁺、低N、P质量浓度和高光强条件下均出现了群体.D₅₀最大分别为37.45、38.22、23.15和36.78μm.相比各对照组·高Ca²⁺、低N,低P、强光和低温条件使结合型胞外聚合物(bound extracellular polymeric substances,B-EPS)含量最大分别升高了54.77%、50.33%、33.06%、59.80%和 67.87%；Zeta 电位最高分别升高47.65%、24.79%、27.61%.8.11%,43.49%.表明这些环境因子可能通过刺激铜绿微囊藻分泌更多的B-EPS、升高Zeta电位等途径改变细胞表面特性，降低细胞间排斥作用来促进铜绿微囊藻聚集.

Colony formation is crucial for the dominance of Microcystis species and blooms. The research investigated the impact of Ca^2＋ concentrations, nitrogen(N) and phosphorus(P) concentrations as well as temperatures and light intensities on M. aeruginosa colony formation and elucidated the underlying mechanisms, The results demonstrated that Microcystis colo-nies appeared under high Ca^2＋ concentration, low N and P concentration, high light intensity and low temperature conditions,with maximum D₅₀ values of 37.45, 38.22, 23.15, and 36.78μm, respectively. Compared to the control groups, high Ca^2＋,low N and P. high light intensity, and low-temperature conditions increased the content of bound extracellular polymerie sub-stances (B-EPS) by 54.77%. 50.33%. 33.06%, 59.80%, and 67.87%, respectively, and elevated the Zeta potentials by 47.65%, 24.79%. 27.61%. 8.11 %. and 43.49%. respectively. These findings suggest that these environmental factors may promote the aggregation of M. aeruginosa by stimulating the secretion of B-EPS, increasing the surface Zeta potentials, and al-tering cell surface properties, thereby reducing intercellular repulsion.

作者：

张萍，秦彩红，朱雅芝，熊朝，陈雪梅

Ping, Qin Caihong, Zhu Yazhi, XiongZhangChao, Chen Xuemei

机构地区：

贵州民族大学生态环境工程学院

引用本文：

张萍，秦彩红，朱雅芝等，环境因子介导细胞表面特性解析铜绿微囊藻群体形成机制[J].河南师范大学学报(自然科学 版),2026,54(4):9-15. (Zhang Ping, Qin Caihong, Zhu Yazhi,et al. Deciphering the mechanism of Microcystis aeruginosa colony formation mediated by environmental factors via cell surface property analysis[J].Journal of Henan Normal University (Natural Science Edition), 2026, 54(4):9-15. DOI:10.16366/j.cnki.1000-2367.2025.04.28.0001.)

基金：

国家自然科学基金

关键词：

蓝藻水华;铜绿微囊藻;群体;环境因子;聚集机制

nanocomposite materials; cascade reaction; combination therapy; anti-tumor

分类号：

X173

环境因子介导细胞表面特性解析铜绿微囊藻群体形成机制.pdf