同源与异源淇河鲫消化酶活性和肝胰脏营养成分的比较分析

摘要：

淇河鲫具有雌核发育和有性生殖两种繁育方式，然而对这两种繁育方式所产生后代的差异却知之甚少.以同源鱼(淇河鲫)和异源鱼(黄河鲤)精子刺激淇河鲫卵子产生的后代为研究对象，比较两类淇河鲫消化酶活性及肝胰脏营养成分的差异.结果显示，同源鲫中肠和肝胰脏的淀粉酶活性显著高于异源鲫，而后肠的淀粉酶活性却显著低于异源鲫;同源鲫前、中、后肠的胰蛋白酶活性均显著低于异源鲫;异源鲫前肠和中肠的脂肪酶活性显著高于同源鲫，而后肠和肝胰脏的脂肪酶活性却显著低于同源鲫.同源鲫和异源鲫中肠的淀粉酶和前肠的胰蛋白酶活性均显著高于其他组织;而同源鲫和异源鲫脂肪酶活性最高的组织分别是肝胰脏和前肠.同源鲫肝胰脏的灰分含量显著大于异源鲫，而水分、粗蛋白、粗脂肪、碳水化合物含量及总能量值与异源鲫无显著性差异.综上，异源鲫的胰蛋白酶活性强于同源鲫，而淀粉酶活性和脂肪酶活性在同源鲫和异源鲫中表现出组织差异性。

Qihe crucian carp(Carassius auratus) can reproduce via either gynogenesis or sexual reproduction, but limit inlormation about the physiological differences between the offspring produced by these two breeding modes is known. In this study, the digestive enzyme activities and hepatopancreas nutrient compositions between homologous fish produced by sperm stimulation of Qihe crucian carp and heterologous fish produced by sperm stimulation of Yellow River carp (Cyprinus carpio haematopterus) were compared. Results showed that the amylase activities in midgut and hepatopancreas of homologous fish were significanty higher than that of heterologous fish; however, heterologous fish showed significantly higher amylase activi-ties in hindgut when compared with homologous fish. The trypsin activities in foregut, midgut and hindgut of homologous fish were significandly lower than that of heterologous fish. For lipase, the homologous fish showed higher activities in hindgut and hepatopancreas but lower activities in foregut and midgut when compared with heterologous fish. The ash content in hepatopan-creas of homologous fish was significantly higher than that of heterologous fish; however, moisture, crude protein, crude lipid, carbohydrate content and total energy value in hepatopancreas showed no significant differences between homologous and heterologous fish. In summary, the trypsin activity of heterologous fish was higher than that of homologous fish, while amyl-ase activity and lipase activity varied among different tissues of homologous and heterologous fish.

作者：

石西，陈思宇，刘相伟，陈锋青，马晓，吴利敏，田雪，李学军

Shi Xi, Chen Siyu, Liu Xiangwei, Chen Fengqing,Ma Xiao, Wu Limin, Tian Xue, Li Xuejun

机构地区：

河南师范大学水产学院；河南省丹江口水库水域生态系统野外科学观测研究站

引用本文：

石西，陈思宇，刘相伟等。同源与异源淇河鲫消化酶活性和肝胰脏营养成分的比较分析[J].河南师范大学学报(自然科学版).2026,54(3);130-135. (Shi Xi. Chen Siyu, Liu Xiangwei, et al. Comparative analysis of digestive enzyme activities and hepatopancreas nutrient compositions between homologous and heterologous Qihe cruciancarp(Carassius auratus) [J]. Journal of Henan Normal University (Natural Science Edition), 2026, 54 (3):130-135.DOI:10.16366/j.cnki.1000-2367.2023.09.17.0001.)

基金：

国家社会科学基金；河南省科技攻关项目；河南省现代农业产业技术体系建设专项基金；河南师范大学博士科研启动基金

关键词：

淇河鲫；同源；异源；营养成分；消化酶

Qihe crucian carp; homologous; heterologous; nutrient composition; digestive enzyme

分类号：

S917.4

同源与异源淇河鲫消化酶活性和肝胰脏营养成分的比较分析.pdf