Proteomic analysis of exosomal proteins in plasma as biomarkers for lung cancer
- 分享到:
摘要：
肺癌是全球范围内最常见且致命的恶性肿瘤之一，其高发病率和死亡率对人体健康构成了严重威胁。为了提高肺癌的早期诊断准确率和治疗效果，需要建立无创或微创,高灵敏度和高特异性的诊断方法。外泌体是一种胞外囊泡，携带多种生物大分子，广泛分布于体液中，并參与细胞间信息交换，对肿瘤的发生发展及侵袭转移有关键作用，可能成为肺癌生物标志物的重要来源,研究结合Ti4+-IMAC 快速分离纯化外泌体方法以及无标记定量蛋白质组学技术，对肺癌患者血浆样本中的外泌体蛋白质组进行了深入分析。结果鉴定出 41个肺癌患者特异性上调或下调表达的血浆外泌体蛋白质，能够有效区分健康人群和肺癌患者。进一步的生信分析揭示，如HDAC3、NAPA、ELP3、HAT1、RAB5B、PDIA5。SARAF等外泌体蛋白质的特异性表达与肺癌的进展显著相关,为肺癌的早期诊断与筛查提供了重要数据支撑。
lung cancer is a prevalent and fatal malignancy globally, which poses a serious threat to public health due to its high incidence and mortality rates, To improve the early diagnosis and treatment outcomes of lung cancer, it is essential to establish minimally invasive, highly sensitive, and specific diagnostic methods, Exosomes are extracellular structures that carry biomacromolecules, They are widely distributed in bodily fluids and play a crucial role in intercellular communication, Exosomes are signilicantly involved in the development, invasion, and metastasis of tumors and may serve as an important source of biomarkers for lung cancer, This study applied label-free quantitative proteomics technology was applied to perform an in-depth analysis of the exosomal proteome in plasma samples from lung cancer patients, The results identified 41 exosomal proteins in the plasma that were either upregulated or downregulated specifically in lung cancer patients, efectively distinguishing them from the healthy population, Subseguent analysis has shown that the particular expresion of exosomal proteins, including HDAC3, NAPA, ELP3, HAT1, RAB5B, PDIA5 and SARAF, is significantly linked to the progression of lung cancer. This provides crucial data to support early diagnosis and screening.
作者：
张勇，易观华，罗海欣，郑雅琳，刘雯婧，顾雨帆，苏涛，曾文娟
Zhang Yong, Yi Guanhua, Luo Haixin, Zheng Yalin, Liu Wenjing, Gu Yufan, Su lao , Zeng Wenjuan
机构地区：
四川大学华西医院华西临床医学院
引用本文：
张勇，易观华，罗海欣等。肺癌血浆外泌体蛋白质组学研究[ ] ].河南师范大学学报(自然科学版),2025,53(4):31-38.(Zhang Yong, Yi Guanhua, Luo Haixin,et al. Proteomic analysis of exosomal proteins in plasma as biomarkers for lung cancer [ J ].Journal of Henan Normal University(Natural Science Edition),2025,53(4):31-38DOI:10.16366/j.cnki.1000-2367.2024.01.30.0001.)
基金：
国家自然科学基金
关键词：
肺癌；血浆；外泌体；生物标志物；质谱；蛋白质组
lung cancer; plasma; exosomes; biomarkers; mass spectrometry; proteomics
分类号：
Q592
