The mechanism of extracts of Cercis Glabra Legumes for treating Alzheimer's disease based on network pharmacology

摘要：

紫荆荚果中含有抑制乙酰胆碱酯酶(acetylcholinesterase,AChE)的有效活性成分,具有潜在治疗阿尔茨海默症(Alzheimer's disease,AD)的作用。以紫荆荚果为研究对象，通过网络药理学方法预测了紫荆荚果治疗AD的作用机制，并对筛选出的化合物的抑制效果进行了评价。结果显示，化合物C4和C13 的活性较显著，可能通过 ESR1、CDK2和 KDR 等核心靶点来调控 PI3K-AKT,MAPK、AGE-RAGE 等信号通路发挥抗 AD 作用。在体外AChE的抑制实验中,化合物 C4(IC₅₀=0.488 mg/mL)和 C13(IC₅₀=0.391 mg/mL)对 AChE具有显著的抑制作用，抑制率达到 68%以上(阳性对照的抑制率为61.44%),其IC₅₀值接近阳性对照品(多奈哌齐 IC₅₀=0.096 mg/mL).实验结果表明紫荆荚果提取物在治疗AD方面具有潜在的应用价值，网络药理学预测了紫荆荚果抗 AD作用机制方面的有效性。

The Cercis Glabra Legumes(Chinese redbud) contains active ingredients that effectively inhibit acetylcholinesterase(AChE), which has a potential therapeutic role in Alzheimer's disease( AD). The mechanism of Cercis Glabra Legumes in the treatment of AD was investigated using a network pharmacology approach, and the inhibitory effect of the selected compounds was evaluated, The results showed that compounds C4 and C13 exhibited significant activities, suggesting that the signaling pathways such as PI3K-AKT, MAPK and AGE-RAGE could be regulated through core targets such as ESR1, CDK2 and KDR to exert anti- AD roles. In vitro AChE imhibition assays revealed that compounds C4(IC₅₀ =0,488 mg/ml) and C13(IC₅₀=0.391 mg/ml) significantly inhibited acetylcholinesterase, with inhibition rates exceeding 68%(compared with a positive control inhibition rate of 61.44%). Furthermore, the IC₅₀, value was close to that of the positive control substance(Donepezil IC₅₀ =0.096 mg/ml). These findings demonstrate the potential application of extracts of Cercis Glabra Legumes in the treatment of AD and the effectiveness of network pharmacology in predicting the ant-AD mechanisms as well.

作者:

曹桦强，徐婷，文思淼，舒朋华，张彭湃

Cao Huaqiang,Xu Ting,Wen Simiao,Shu Penghua,Zhang Pengpai

机构地区：

河南大学生命科学学院；河南大学河南省应用微生物工程研究中心；许昌学院食品与药学院

引用本文：

曹桦强，徐婷，文思淼等。基于网络药理学探讨紫荆荚果提取物治疗阿尔茨海默症的作用机制[J].河南师范大学学报(自然科学版),2025,53(6):101-108.(Cao Huagiang,Xu Ting, Wen Simiao,et al. The mechanism of extracts of Cercis Glabra legumes for treating Alzheimer's disease based on network pharmacology[J].Journal of Henan Normal University(Natural Science Edition),2025,53(6):101-108.DOI:10.16366/j.cnki.1000-2367.2024.09.23.0002.)

基金：

国家自然科学基金；河南省科技攻关计划项目；河南省高校重点科研计划项目

关键词：

紫荆荚果；网络药理学；乙酰胆碱酯酶；阿尔兹海默症；作用机制

Cercis Glabra Legumes; network pharmacology; acetylcholinesterase; Alzheimer's disease; action mechanism

分类号：

R285

基于网络药理学探讨紫荆荚果提取物治疗阿尔茨海默症的作用机制.pdf