The interaction of histone H2A.W and AGO2 in Arabidopsis thaliana

摘要：

以拟南芥AGO2为研究对象,通过免疫共沉淀质谱联用技术(IP-MS),发现了HTA6和HTA7可能与AGO2蛋白互作,并通过亚细胞定位、双分子荧光互补技术以及免疫共沉淀(Co-IP)实验,进一步研究了H2A.W家族蛋白HTA6和HTA7与AGO2蛋白的定位和相互作用关系.结果显示HTA6,HTA7和 AGO2在细胞核内有共定位,且HTA6,HTA7分别与AGO2在细胞核内存在相互作用，体内Co-IP实验也进一步证明了HTA6.HTA7和AGO2的互作关系，为后续研究AGO2蛋白与异染色质之间的调控关系奠定了基础.

Taking Arabidopsis thaliana AGO2 as the research object, through the immunoprecipitation-mass spectrom-etry(IP-MS) technique, HTA6 and HTA7 were found to potentially interact with AGO2 protein. Further studies on the locali-zation and interaction relationship between H2A. W family protcins HTA6 and HTA7 and AGO2 protcin were conducted using subcellular localization, bimolecular fluorescence complementation technology and co-immunoprecipitation(Co-IP) experiments.The results showed that HTA6, HTA7 and AGO2 were co-localized in the nucleus, and HTA6 and HTA7 respectively inter-acted with AGO2 in the nucleus. The in vivo Co-IP experiment further confirmed the interaction relationship among HTA6.HTA7 and AGO2, laying a foundation for subsequent research on the regulatory relationship between AGO2 protein and heter ochromatin.

作者: 

杨献光,徐珑倩,刘洋

Yang Xianguang, Xu Longqian, Liu Yang

机构地区：

中国科学院动物研究所；农业虫害鼠害综合治理研究国家重点实验室

引用本文：

杨献光，徐珑倩，刘洋。拟南芥中组蛋白H2A.W与AGO2蛋白的互作研究[J].河南师范大学学报(自然科学版).2026,54(3) : 136-142. (Yang Xianguang. Xu Longqian. Liu Yang. The interaction of histone H2A. W and AGO2 in Arabidopsis thaliana [J].Journal of Henan Normal University(Natural Science Edition),2026,54(3):136-142.DOI:10.16366/j.cnki.1000-2367.2024.02.21.0003.)

基金：

国家社会科学基金

关键词：

组蛋白;H2A;AGO2;互作

histone; H2A; AGO2; interaction

分类号：

Q71

拟南芥中组蛋白 H2A.W 与 AGO2蛋白的互作研究.pdf