姓名:史西保 | |||
职称:副教授,硕士生导师 | |||
办公电话: | |||
电子邮箱:shixibao@aliyun.com | |||
个人简介: |
预防兽医学博士,学术上,主要从事猪繁殖与呼吸综合征病毒(PRRSV)天然免疫学、病毒快速检测和疫苗学研究。以第一作者在国际著名期刊Journal of virology,FASEB J,Cell Mol Immunol, Viruses-BaseL等发表SCI论文20余篇,其中,SCI 2区以上8篇。 参加工作以来,主持国家自然科学基金1项、河南省自然基金1项,农业部重点实验开放课题1项、河南师范大学优秀青年培育项目1项和省教育厅项目1项,河南省科技特派员项目1项。受理和授权国家发明专利5项;合作出版专著2本。 教育经历 2011/9 - 2014/7,河南农业大学,预防兽医学,博士,导师:张改平 2005/9 - 2008/12,中国科学技术大学,生物化学与分子生物学,硕士,导师:陈小平 2001/9 - 2005/7,河南师范大学,生物科学,学士 科研与学术工作经历 2014/7 - 至今,河南师范大学,生命科学学院,副教授 2008/12 - 2014/7,河南省农业科学院,河南省动物免疫学重点实验室,助理研究员 |
研究领域: |
病毒天然免疫学、病毒快速检测和病毒疫苗研究 |
主要学术及社会兼职: |
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主持或参加科研项目情况: |
1.国家自然基金青年科学基金项目,31302073,猪繁殖与呼吸综合征病毒(PRRSV)非结构蛋白(nsp)1α和nsp11抑制β干扰素转录的分子机理研究,2014/01-2016/12,25万元,结题,主持。 2.河南省自然科学基金,182300410077,抑制猪繁殖与呼吸综合征病毒(PRRSV)复制的宿主蛋白筛选及其机理研究,2018/01-2019/12,10万,在研,主持。 3.农业部重点实验室开放课题,PKLAI20170605,miR-376b-3p促进猪繁殖与呼吸综合征病毒(PRRSV)复制的分子机理研究,2017.6-2019.6,10万元,结题,主持。 4.河南师范大学2016年度优秀青年科学基金项目,miR-376b-3p促进猪繁殖与呼吸综合征病毒(PRRSV)复制的分子机理研究,2017.6-2020.6,15万元,结题,主持。 5.河南省高等学校重点科研项目,17A180006,核因子IA(NFIA)抑制猪繁殖与呼吸综合征病毒(PRRSV)复制的分子机理研究,2017/01-2018/12,5万元,结题,主持。 6.国家自然科学基金面上项目,31472177,猪繁殖与呼吸综合征病毒(PRRSV)结构蛋白T细胞表位图谱的绘制,2015/01-2018/12, 88万元,结题,参加。 |
学术成果: |
代表性论文: (1)Xibao Shi, Xiaomin Fan, Shouyi Nie, Liyun Kou, Xiaozhuan Zhang, Hongliang Liu, Shengdong Ji, Ruiguang Deng, Aiping Wang2, Gaiping Zhang, Identification of a linear B-cell epitope onglycoprotein (GP)2a of porcine reproductive and respiratory syndrome virus(PRRSV). Int J Biol Macromol. 2019 Jul 26;139:1288-1294. doi: 10.1016/j.ijbiomac.2019.07.171.(IF2021=6.953,SCI 2区) (2)Xibao, Shi, Yuanhao Yang, XiaozhuanZhang, Xiaobo Chang, Jing Chen, Chao Wang, Aiping Wang, Jianhua Wang, Jianru Qin, Xianlong Ye, Wei Jin, and Gaiping Zhang. 2022. miR-541-3p Promoted Porcine Reproductive and Respiratory Syndrome Virus 2 (PRRSV-2) Replication by Targeting Interferon Regulatory Factor 7 Viruses 14, no. 1: 126.https://doi.org/10.3390/v14010126.(IF2021=5.048, SCI 2区). (3)Chen jing,Shi xibao(共同一作及通讯作者),Zhang xiaozhuan,Aiping Wang, Li Wang, Yanyan Yang, Ruiguang Deng, zhang gaiping(*),MicroRNA 373 Facilitates the Replication of Porcine Reproductive and Respiratory Syndrome Virus by Its Negative Regulation of Type I Interferon Induction,Journal of Virology,2017.01.01,91(3):e01311~e01316 (IF2021= 5.103, SCI 2区,top 期刊) (4)Xiaobo Chang, Xibao Shi(共同一作), Xiaozhuan Zhang, Jing Chen,Xiaomin Fan, Yuanhao Yang, Li Wang, Aiping Wang, Ruiguang Deng, Enmin Zhou, Gaiping Zhang, miR-382-5p promotes Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) replication by negatively regulating the inductionof type I interferon, FASEB J https://doi.org/10.1096/fj.201902031RRR,2020. 34(3):4497-4511.(IF2018=5.391,SCI 2区),. (5)Xiaobo Chang, Xibao Shi(共同一作), Xiaozhuan Zhang, Jing Chen,Xiaomin Fan, Yuanhao Yang, Li Wang, Aiping Wang, Ruiguang Deng, Enmin Zhou, Gaiping Zhang, IFI16 inhibits porcine reproductive and respiratory syndrome virus (PRRSV) replication by its positively regulating MAVS signaling pathway. Viruses-BaseL,2020.11(12), 1160.(IF2018=3.811,SCI 2区) (6)Shi xibao,Zhang xiaozhuan,Wang li,Zhang gaiping,Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promoted PRRSV replication in MARC-145cells,BMC Veterinary Research. 2016. 12 (IF2018=1.792, SCI 2区). (7)Shi xibao,Xiaozhuan Zhang,Li Wang,Weiguo Li,Bo Jiang,Ruiguan Deng,Aiping Wang,Gaiping Zhang,Recombinant beta interferon could clear the low-dose infected porcine reproductive and respiratory syndrome virus (PRRSV) but not to the high-dose infected PRRSV in MARC-145 cells,ACTA VIROLOGICA,60(3), 290-297. (8)Wang, Chao,Shi, Xibao(共同一作及通讯作者),Zhang, Xiaozhuan,Wang, Aiping,Wang, Li,Chen, Jing,Deng, Ruiguang,Zhang,Gaiping,The Endoribonuclease Activity Essential for the Nonstructural Protein 11 of Porcine Reproductive and Respiratory Syndrome Virusto Inhibit NLRP3 Inflammasome-Mediated IL-1beta Induction.,DNA and Cell Biology(IF2018=2.918),2015.12.01,34(12):728~735 (9)Chen, Jing,Shi, Xibao(共同一作),Zhang, Xiaozhuan,Wang, Li,Luo, Jun,Xing, Guangxu,Deng, Ruiguang,Yang, Hong,Li, Jinting,Wang, Aiping,Zhang, Gaiping,Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Inhibits RNA-Mediated Gene Silencing by Targeting Ago-2.,Viruses-BaselL (IF2018=3.811, SCI 2区),,2015.10.01,7(10):5539~5552 (10) Xibao Shi,Yongzhe Chang,Xiaozhuan Zhang,Li Wang,Chunxi Li,Kai Jiang,Jing Chen,Chao Wang,Ruiguang Deng,Jianming Fan,Gaiping Zhang(*),Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells,Research in Veterinary Science(IF2018=1.751),2015.4.01,66:215~217 (11)Shi, Xibao,Zhang, Xiaozhuan,Wang, Fangyu,Wang, Li,Qiao, Songlin,Guo, Junqing,Luo, Chunhui,Wan, Bo,Deng, Ruiguang,Zhang, Gaiping,The Zinc-Finger Domain Was Essential for Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein-1 alpha to Inhibit the Production of Interferon-beta,Journal of Interferon and Cytokine Research(IF2018= 1.774),2013.6.01,33(6):328~334 (12)Shi, Xibao,Chen, Jing,Xing, Guangxu,Zhang,Xiaozhuan,Hu, Xiaofei,Zhi, Yubao,Guo, Junqing,Wang, Li,Qiao, Songlin,Lu, Qingxia,Zhang,Gaiping,Amino acid at position 176 was essential forporcine reproductive and respiratory syndrome virus (PRRSV) non-structural protein 1 alpha (nsp1 alpha) as an inhibitor to the induction of IFN-beta,CELLULAR IMMUNOLOGY (IF2018=3.291),2012.12.01,280(2):125~131 (13)Shi, Xibao,Wang, Li,Li, Xuewu,Zhang,Gaiping,Guo, Junqing,Zhao, Dong,Chai, Shujun,Deng,Ruiguang,Endoribonuclease activities of porcine reproductive and respiratory syndrome virus nsp11 was essential for nsp11 to inhibit IFN-beta induction,MOLECULAR IMMUNOLOGY (IF2018=3.064),2011.7.01,48(12-13):1568~1572 (14)Shi, Xibao,Zhang,Gaiping,Wang, Li,Li, Xuewu,Zhi, Yubao,Wang, Fangyu,Fan, Jianming,Deng,Ruiguang,The Nonstructural Protein 1 Papain-Like Cysteine Protease Was Necessary for Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1 to Inhibit Interferon-beta Induction,DNA AND CELL BIOLOGY(IF2018=2.918),2011.6.01,30(6):355~362 (15)Shi, Xibao,Wang, Li,Zhi, Yubao,Xing, Guangxu,Zhao, Dong,Deng,Ruiguang,Zhang, Gaiping,Porcine reproductive and respiratory syndrome virus(PRRSV) could be sensed by professional beta interferon-producing system and had mechanisms to inhibit this action in MARC-145 cells,VIRUS RESEARCH(IF2018=2.736),2010.10.01,153(1):151~156 (16)Shi, Xibao,Qin, Li,Liu, Guangjie.,Zhao, Siting,Chen, Xiaoping.,Dynamic balance of pSTAT1 and pSTAT3 in C57BL/6 mice infected with lethal or nonlethal Plasmodium yoelii,Cell Mol Immunol (IF2018=8.231 SCI 2区),2008.8.20,5(5):341~348 (17)Chang, Li,Zhang,Xiaozhuan,Shi, Xibao,Zhao, Liang,Liu, XuanMing,Synthesis and Characterization of a Novel Fibrous Antibacterial Fiber with Organophorsphor Functional Groups,Journal of Applied Polymer Science,2014.10.15,131(20) (18)Chang, Li,Zhang,Xiaozhuan,Shi, Xibao,Zhao, Liang,Liu, Xuanming,Preparation and Characterization of a Novel Antibacterial Fiber Modified by Quaternary Phosphonium Salt on the Surface of Polyacrylonitrile Fiber,Fibers and Polymers,2014.10.01,15(10):2026~2031 (19)Chen Lili,Qin Li,He Zhengxiang,Li Qinyan,shi xibao,Chen xiaoping,Antitumor effect of malaria parasite infection in a murine Lewis lung cancer model through induction of innate and adaptive immunity. PLoS One,2010.01.01,6,(e24407.):24407~e24407 (20)Li, Xuewu,Wang, Li,Shi, Xibao,Zhao, Dong,Yang, Jifei,Yang, Suzhen,Zhang, Gaiping,Development of an immunochromatographic strip for rapid detection of antibodies against classical swine fever virus,Journal of Virological Methods,2012.3.01,180(1-2):32~37 (21)Bao, Dengke,Wang, Rui,Qiao, Songlin,Wan, Bo,Wang, Yinbiao,Liu, Mingyang,Shi, Xibao,Guo, Juiqing,Zhang, Gaiping,Antibody-dependent enhancement of PRRSV infection down-modulates TNF-alpha and IFN-beta transcription in macrophages,Veterinary Immunology and Immunopathology,2013.11.15,156(1-2):128~134 (22)赵慧君, 赵旭阳, 史西保, 陈静, 范小敏, 司朝朝, 王丽, 李青梅, 常晓博, 李雪莹, 付建蒙, 徐雅甜, 邓瑞广, 张改平,猪繁殖与呼吸综合征病毒非结构蛋白1α多克隆抗体的制备及应用,《安徽农业科学》 2019年第12期115-119 (23)车志芬, 史西保, 张改平, 扣莉云, 周景明, 祁艳华, 王爱萍,猪繁殖与呼吸综合征病毒BJ-4株GP4蛋白的原核表达与纯化。《西北农林科技大学学报:自然科学版》, 2017。第5期7-14 (24)王超(#),史西保,王丽,陈静,司朝朝,王爱萍,邓瑞广,张改平(*),猪繁殖与呼吸综合征病毒非结构蛋白1α(nsp1α)的N端锌指结构是其抑制NLRP3炎症小体活性所必需,畜牧兽医学报,2015.11.01,(11):2032~2039 (25)范璐,王丽,史西保,樊剑鸣,王爱萍,张改平*,.12. 2014,稳定表达靶向BVDV shRNA细胞系的建立及其对BVDV复制的影响,河南农业科学,2014.12.15,43(12):134~139 (26)司朝朝,史西保,李恭贺, 乔松林, 王丽,李青梅,王超,陈静, 王寅彪,张改平(*),猪繁殖与呼吸障碍综合征病毒非结构蛋白nsp1α的表达及活性检测,.河南农业科学,2015.1.15,44(1):126~129 (27)王大伟, 李学伍*., 王丽 , 史西保.,猪伪狂犬病毒的增殖、纯化和鉴定,河南农业科学,2010.11.15,40(11):125~127 (28)刘明,张改平,肖治军,郭军庆,乔松林,王丽,史西保,刘运超,游雷鸣,王爱萍(*),PRRSV河南株ORF7基因的克隆表达及条件优化,河南农业科学,2011.01.01,(01):136~140 (29)王丽,杜晓明,王林林,史西保,藤蔓,李功权,权凯,郭军庆,张改平(*),人热休克蛋白10和鼠热休克蛋白10的克隆、序列分析及其原核表达,华北农学报,2011.01.01,(03):56~59 赵慧君,赵旭阳,史西保,陈静,范小敏,王丽,李青梅,常晓博,邓瑞广,张改平。甲基-CpG 结合结构域蛋白 2 抑制 PRRSV 复制的研究。《河南农业大学学报》,2019.53(6),903-911 (30)赵旭阳,史西保,张小转,陈静,王丽,邓瑞广,张改平。核因子I/A(NFIA)依赖其N末端的DNA结合结构域抑制猪繁殖与呼吸综合征病毒(PRRSV)复制。《中国兽医学报》,2020,40(6),1087-1094 会议论文: (1)Shi,X., Li, Q., Chang,Y., Chen, J., Wang, C., Zhao, P., Yang, J., Yang, S., Zhang, G*., The nonstructural protein 1 papain-like cysteine protease was not necessary forporcine reproductive and respiratory syndrome virus nonstructural protein 1 to inhibit interferon-beta function. 2013 International PRRS Symposium and PCVAD(PCV2) ,MAY 20-22 2013, 122, BeiJing, 2013, http://www.prrssymposium.org/Documents/Proceedings/PrrsSymposiumProceedings2013.PDF, (2)史西保,常永哲, 王方雨,王丽, 乔松林,郭军庆, 陈静,王超,张改平.N端锌指结构是猪繁殖与呼吸综合征病毒(PRRSV)nsp1α抑制β干扰素转录所必需.2013年全国动物疫病与食品安全博士后学术论坛,(2013.11.6-9) , 34. 河南郑州, (2013) (3)史西保,李青梅, 常永哲,陈静, 王超,赵朴, 杨继飞,张改平. 木瓜样蛋白酶活性不是猪繁殖与呼吸综合征病毒非结构蛋白1抑制β干扰素作用所必需. 2013年全国动物疫病与食品安全博士后学术论坛, (2013.11.6-9) , 35. 河南郑州, (2013) (4)史西保,常永哲, 邢广旭,陈静, 王超,王方雨, 王丽,乔松林, 郭军庆,张改平. PRRSV抑制Ⅰ型干扰素转录及其分子机理探讨. 长三角“动物疾病防控与食品安全”研究生论坛,2013.11.15-17,江苏扬州 合作译著与专著: (1)合作专著《免疫层析试纸快速检测技术》,河南科学技术出版社(2015年) (2)合作译著:《兽医免疫学》,中国农业出版社,第8版 (2012年) 获奖情况 (1)史西保(5/10)猪繁殖与呼吸综合征病毒感染和免疫分子机制, 河南省人民政府,河南省自然科学奖,一等奖,2020. 合作国家发明专利: (1)马立克氏病病毒和J亚群禽白血病病毒快速联检试纸条,中国发明专利,CN103235129A (2)禽网状内皮组织增生病病毒和J亚群禽白血病病毒快速联检试纸条,中国发明专利,CN103235128A 承担课程: 《生物工程制药》、《实验动物学》 |